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. 2008 Mar 7;4(3):e1000021. doi: 10.1371/journal.ppat.1000021

Figure 4. Activated Abl kinase is recruited to EBs during entry.

Figure 4

HeLa cells were pretreated with DMSO (panels A–D), STI571 (panels E–H), or AG1295 (panels I–L) for 1 hour and subsequently infected with C. trachomatis in the presence of carrier or the indicated drug. The cells were fixed and stained with antibody that recognizes endogenous Abl kinase (panels A, E, and I; red in merge) and an antibody that recognizes phospho-Abl Y412 (panels B, F, and J; green in merge). Bacteria and host DNA were detected using DAPI (panels C, G, and K; blue in merge). The merged panels are shown on the right (panels D, H, L). The exposure time for each filter of all images was identical. PDGFR activity is not required for recruitment and activation of Abl kinase.