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. 2008 Mar 7;4(3):e1000021. doi: 10.1371/journal.ppat.1000021

Figure 7. Abl kinase is sufficient for C. trachomatis entry.

Figure 7

HeLa cells were transfected with Abl kinase (Abl WT) or an STI571 resistant allele of Abl kinase (Abl STI571R), treated with STI571, and then infected with C. trachomatis for 1 hour. (A) Cells were fixed and stained with an antibody that recognizes phosphorylated Abl kinase (green) and DAPI to identify bacteria (blue). The exposure time for each filter of all images was identical. Transfection of Abl STI571R (panel D) but not wild type Abl (panel B) permits recruitment of phospho-Abl in the presence of STI571. (B) A parallel set of cells were fixed, stained, and analyzed for C. trachomatis internalization efficiency as described in Materials and Methods. The internalization efficency (mean±s.e.m.) is the percentage of internalized EBs/total cell-associated EBs. Data are representative of three independent experiments. Samples are normalized to DMSO-treated cells. Expression of Abl STI571R permits internalization in the presence of STI571. **p<0.01 for STI571-treated Abl WT cells compared to DMSO-treated Abl WT cells. *p<0.05 for STI571-treated Abl WT cells compared to STI571-treated Abl STI571R cells (ANOVA).