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. 2008 Mar 7;4(3):e1000021. doi: 10.1371/journal.ppat.1000021

Figure 8. Abl kinase and PDGFR signal to overlapping downstream effectors.

Figure 8

HeLa cells were transfected with control or Abl kinase siRNA for 48 hours, treated with DMSO or AG1295 for 1 hour, and then subsequently infected with C. trachomatis for 1 hour. WAVE2 and Cortactin were immunoprecipitated from lysates and subjected to western blot analysis with 4G10 to assess phosphorylation. Blots were reprobed for total protein amounts. Lysates from the same set of samples were probed with anti-pVav2, total Vav2, total Abl, and GAPDH (loading control) antibodies. The percentage of phosphorylated protein compared to total protein was quantified by densitometry analysis and normalized relative to C. trachomatis-infected samples. Immunoblots shown are representative of three independent experiments. C. trachomatis induces phosphorylation of WAVE2, Vav2, and Cortactin (lane 2). Inhibition of PDGFR or Abl signaling decreases WAVE2, Vav2, and Cortactin phosphorylation.