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. 2008 Mar 13;118(4):1491–1501. doi: 10.1172/JCI33467

Figure 5. Adenosine stimulates an increase in both cAMP and cGMP in CCSMCs via A2BR activation.

Figure 5

(A) Adenosine receptor mRNA expression profile in purified primary CCSMCs were determine by quantitative real-time RT-PCR. nd, not determined. (B) cAMP levels of CCSMCs from wild-type mouse penes in the presence of different concentrations of adenosine with or without specific adenosine receptor agonists or antagonists. Data are means ± SEM. *P < 0.05 versus adenosine alone. (C) cAMP levels of CCSMCs from wild-type and A2BR–/– mice treated with adenosine, NECA, or forskolin. Data are means ± SEM (n = 4). *P < 0.05 versus untreated wild-type; **P < 0.05 versus untreated A2BR–/–. (D) cGMP levels in CCSs of wild-type and A2BR–/– mice treated with l-NAME or MRS1706. Data are means ± SEM (n = 6–7). *P < 0.05 versus wild-type treated with adenosine alone. (E) cGMP levels in CCSMCs of wild-type and A2BR–/– mice treated with the indicated compounds. Data are means ± SEM. *P < 0.05 versus respective control.