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Time course of phosphate release (μmoles per mg protein) on ATP hydrolysis at 23°C under Na,K-ATPase assay conditions (130 mM NaCl, 20 mM KCl, 4 mM MgCl2, 3 mM ATP, 30 mM histidine, pH 7.4) for enzyme suspended in assay buffer (▴), in assay buffer with 20% PEG (▪), or with 20% glycerol (•).
