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PCH analysis of Paxillin-EGFP
| Adhesion | Cytoplasm | |
|---|---|---|
| ɛ1 (in c/s/m) | 5,700 | 5,730 |
| N1 | 4.57 | 5.61 |
| ɛ2(in c/s/m) | 122,240 | 68,480 |
| N2 | 0.23 | 0.049 |
| ɛ2/ɛ1 | 21.4 | 12 |
| N2 (as % of total) | 4.8 | 0.9 |
The PCH curves were Globally fitted to a two species model where the molecular brightness (ɛ) and number of molecules (N1 and N2) were allowed to vary. The percent number of molecules for the brighter species (N2) shows to be more abundant in the adhesions versus the cytosol.
