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Energy landscape Ej of a 1000-bp DNA fragment taken from the λ-bacteriophage sequence for three values of the bias parameter W. At j = 0 the duplex is fully closed and the single-stranded overhang is advanced as far as possible into the pore. Negative j corresponds to an entirely closed duplex diffused backward by j nucleotides relative to the j = 0 configuration. Positive j-values describe duplexes with j unzipped basepairs diffused forward by j nucleotides. T = 20°C.
