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. 2007 Dec 7;94(7):2492–2504. doi: 10.1529/biophysj.107.113050

FIGURE 1.

FIGURE 1

The process of particle recognition and identification. All images are captured from processed movies and at the same frame in the movies. Blowups are smoothed by the texture smoothing mechanism of OpenGL to ease the jaggyness. (a) The original image converted to grayscale. The smallest rectangle indicates the position of blowups in panels bd and Fig. 2, a and b. The middle rectangle indicates the position of blowups in Fig. 2, c and d. The largest rectangle indicates the position of Fig. 9. (b) Blowup of the enhanced image. Darker pixels are now occupied by moving particles and lighter pixels are previously occupied but now free of particles. The open polygons indicate the recognized clusters. The smallest rectangles correspond to one pixel. (c) Trajectories of the center of gravity of the recognized clusters. (d) The velocity of recognized clusters. To avoid the misidentification, the velocity is calculated from last four trajectory points. The underneath plasmodium image is blowup of the original image.