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Specific binding of N21-30:Fc to a Notch protein including EGF repeats 11–12. A. N1-10:Fc did not bind to N11-20. B. N11-20:Fc did not bind to N11-20.C. N21-30:Fc showed saturable binding to N11-20. Although results vary from one protein batch to the next, in most cases more N21-30:Fc binds than does Dl:Fc (see panel E). D. N31-36:Fc did not show saturable binding to N11-20. E. Dl:Fc binding to N11-20.
