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. 2008 Jan 21;8:4. doi: 10.1186/1471-213X-8-4

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Copyright © 2008 Pei and Baker; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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pH does not affect binding to the spl mutant. A. Dl:Fc bound to adsorbed N11-14:V5:His protein at pH7.4, B. Dl:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH7.4, C. Dl:Fc binding to adsorbed N11-14:V5:His protein was enhanced at pH5.4, D. Dl:Fc binding to adsorbed N11-14spl:V5:His protein was similarly enhanced at pH5.4, E. N21-30:Fc bound to adsorbed N11-14:V5:His protein at pH7.4, F. N21-30:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH7.4, G. N21-30:Fc binding to adsorbed N11-14:V5:His protein was not enhanced at pH5.4, H. N21-30:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH5.4,