Figure 3.

Daughterless is required for the endogenous Dap expression and for the ectopic Dap expression in Su(H) mutant cells. The level of Dap protein (red) is decreased in da mutant clones (A-C) as well as in da, Su(H) double mutant clones (D-F). No Dap-HB reporter activity (red in G-I) was observed in da mutant clones. Although increased Dap-HB reporter expression was detected in the Su(H) single mutant clones (Fig. 2D-F), no increased reporter activity was observed in da, Su(H) double mutant clones (red in J-L). Mutant cells are marked by the absence of GFP.