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. 2008 Jan 22;4:3. doi: 10.1186/1746-4811-4-3

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Copyright © 2008 Dubin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transient expression in Nicotiana benthamiana tobacco leaves. A, Protein extracts (20 μg) from tobacco leaves transiently transformed with pK3nDET1 and pH2nDDB1 either alone or in combination were separated on 10% SDS-PAGE and analysed by western blotting using antibodies against HA and c-Myc as indicated. Ponceau staining is shown as loading control below each panel. B, Immunoprecipitation analysis of protein extracts (200 μg) from leaves transformed with the indicated tagged proteins with c-Myc antibody. Total extracts (30 μg) and immunoprecipitated samples were resolved on 10% SDS-PAGE and probed with α-HA antibody.