Figure 2.

The orb6⁺ gene. (A) Sequence comparison of Orb6 and homologous proteins. An homology search using EMBL and GenBank databases identified S. cerevisiae 1050791, Neurospora crassa cot1 (14), human Ndr (23), Drosophila warts (15, 16), human PK428, Rho-associated kinase (17–19), and human DMPK (20). (B) orb6⁺ gene deletion. Spores were plated on yeast extract plates and allowed to germinate for 16 hr at 32°C. (a) ade- leu1–32 ura4-D18 microcolony. (b) orb6∷ura4⁺ ade- leu1–32 ura4-D18 microcolony. (c) orb6∷ura4⁺ ade- leu1–32 ura4-D18 microcolony after 24 hr at 32°C. (Bar = 5 μm.)