Figure 3.

Orb6⁺ gene dosage effects. orb6∷ura4⁺ ade- leu1–32 ura4-D18 cells containing integrated HA-tagged orb6⁺ were grown exponentially for at least eight generations at 32°C, then thiamine was added to the culture. (a and d) Cells grown in the absence of thiamine. (b and e) 7.5 hr after thiamine addition. (c and f) 12 hr after thiamine addition. Cells were photographed immediately (a–c) or fixed and stained for actin (d–f). (Bar, a–c = 5 μm.; d–f = 10 μm.)