Figure 5.

Overexpression of Wee1 in orb6 mutants and actin localization in orb6–25 and wee1–50 mutants. (a–d) Overexpression of Wee1 in orb6 mutants. (a) leu1–32h⁻ pRep3x. (b) orb6–25 leu1–32h⁻ pRep3x. (c) leu1–32h⁻ pRep3x wee1+. (d) orb6–25 leu1–32h⁻ pRep3x wee1+. (e) wee1–50h⁻. Cells were grown exponentially at 25°C in the presence of thiamine for at least eight generations; thiamine was washed away, and cells were grown in the absence of thiamine at 25°C for 16 hr, then shifted at 36°C for 5 hr. (f–h) Actin localization in orb6–25 and wee1–50 mutants. (f) leu1–32h⁻. (g) orb6–25 leu1–32h⁻. (h) wee1–50h⁻. (Bar = 5 μm.)