Figure 7.

Cloning of the orb2⁺ gene. (a) top, ade6-M210 leu1–32h⁻; left, orb6–25 ade6-M210 leu1–32h⁻; bottom, orb2–34 ade6-M210 leu1–32h⁻; right, orb2–34 orb6–25 ade6-M210 leu1–32 h⁻. Cells were grown at 32°C. (b) ade6-M210 leu1–32h⁻, Rep3X. (c) orb2–34 ade6-M210 leu1–32h⁻, pRep3X. (d) orb2–34 ade6-M210 leu1–32h⁻ pDB248′-8 (genomic fragment). (e) orb2–34 ade6-M210 leu1–32h⁻ pRep3X pak1/shk1⁺. (f) orb2–34 ade6-M210 leu1–32h⁻ pRep3X orb6⁺. Cells were grown exponentially at 25°C in the presence of thiamine for at least eight generations; thiamine was washed away and cells were grown in the absence of thiamine at 25°C for 16 hr, then shifted at 36°C for 6 hr. (Bar = 5 μm.)