Table 1.
Strain | Plasmid | Cell length at division* | % wt length |
---|---|---|---|
(1) leu1-32 | Multicopy plasmid (Rep3X) | 14.6 ± 1.0 | 100% |
(1) leu1-32 | Integrant nmt1 orb6HA | 17.8 ± 1.9 | 121% |
(1) leu1-32 | Multicopy nmt1 orb6HA | 26.6 ± 4.9 | 181% |
(1) leu1-32 | Multicopy nmt1 orb6HA ala122 | 13.7 ± 2.7 | 93% |
(2) leu1-32 | Multicopy plasmid (Rep3X) | 14.2 ± 1.0 | 100% |
(2) leu1-32 | Multicopy nmt1 orb6HA | 28.9 ± 5.0 | 203% |
Δorb6 | Integrant nmt1 orb6HA | 18.0 ± 1.3 | 122% |
Δorb6 | Integrant nmt1 orb6HA (+th., 5h.) | 12.4 ± 0.7 | 84% |
Δorb6 | Integrant nmt1 orb6HA (+th., 7.5h.) | 9.5 ± 0.6 | 65% |
leu1-32 | Multicopy nmt1 orb6HA | 20.0 ± 5.6 | 137% |
wee1-50 leu1-32 | Multicopy plasmid (Rep3X) | 7.4 ± 0.9 | 50% |
wee1-50 leu1-32 | Multicopy nmt1 orb6HA | 7.5 ± 0.9 | 51% |
Thirty cells at division were measured for each sample. The mean values ± SD are shown.