Figure 2. TRPM7 undergoes extensive autophosphorylation of the C-terminus via a sequential series of phosphorylation events.

(A) Autophosphorylation of TRPM7 dramatically reduces its electrophoretic mobility. WT and KD TRPM7-C-long were incubated in the absence or presence of MnATP for 30 min and detected using anti-HA (top panel) and anti-pThr antibodies (bottom panel). (B) Kinetics of TRPM7 autophosphorylation. TRPM7-C-long was incubated with MnATP for the indicated times. Proteins were detected by immunoblotting using anti-HA antibodies.