Figure 6.

Influence of oligopeptide repeats. Unseeded fibre assembly reactions monitored by CR binding: (A) NM^wt (squares), NM^R2E2 (circles) and NM^ΔR2−5 (triangles); and 150 μg/ml each. After completion of the assembly reaction, samples were investigated by AFM: (B) NM^R2E2 and (C) NM^ΔR2−5. Scale bars, 500 nm. AFM, atomic force microscopy; CR, congo red; M, highly charged middle region; N, asparagine- and glutamine-rich region; NM^ΔR2−5, partial oligopeptide repeat deletion mutant NM; NM^R2E2, oligopeptide expansion mutant of NM; NM^wt, wild-type NM.