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. 2007 Oct 12;8(12):1183–1189. doi: 10.1038/sj.embor.7401086

Figure 4.

Figure 4

Tpp2 heterozygous mutant mice are lean. (A) Schema of the wild-type (WT) Tpp2 locus and the mutant (Mut) Tpp2 allele. Splice acceptor (SA) and the lacZ-neomycin selectable marker (β-geo) cassette were inserted and disrupt gene expression from the third exon (E3). (B) Western blot showing reduction of TPPII protein in GWAT from Tpp2 heterozygous mouse compared with wild-type control. (C) Genomic DNA was extracted from pups of multiple Tpp2 heterozygous (het) intercrosses and then genotyped for the presence of the wild-type and mutant Tpp2 alleles. The data indicate that Tpp2 homozygous (homo) mutant mice die in utero. (D) Average body weight (left panel) and average body fat percentage (right panel) of 16-week-old Tpp2 heterozygous mice (n=15) and control (Cont) littermates (n=14). (E) Photograph of representative gonadal WAT of control and Tpp2 heterozygous littermates. (F) Average weights of IWAT, GWAT and MWAT from Tpp2 heterozygous mice (n=5) and wild-type littermate controls (n=7). (G) Average weights of liver, heart, spleen and kidney isolated from the mice in (F). (H) Histological sections of control and Tpp2 heterozygote GWAT and IWAT. (I,J) Plasma of control (n=5) and Tpp2 heterozygous (n=5) mice were analysed for insulin (I) and leptin (J) levels. (K) Food intake of wild-type (n=6) and Tpp2 heterozygous mice (n=5) was measured daily for 1 week, averaged and then plotted. Error bars indicate s.d.; *P<0.05; **P<0.01; NS, not significant by t-test. GWAT, gonadal white adipose tissue; IWAT, inguinal white adipose tissue; MWAT, mesenteric white adipose tissue; TPP, tripeptidyl peptidase.