Figure 1.

The insulin-stimulated glucose and palmitate transport in (immortalized cardiomyocytes) HL-1 cells. (a) Glucose and palmitate uptakes were measured radiochemically as described in Methods. Insulin stimulates in a concentration-dependent manner the rate of both transports. Insulin potency (EC₅₀) was calculated by the fitting of experimental points. (b) Pre-incubation of cells with wortmannin reduced the insulin stimulation of glucose and palmitic acid uptake. Results are presented as stimulated rate compared to the basal transport rate, taken as 100, and they represent the mean±s.e.mean of at least eight (a) or six (b) experiments run in triplicate. For the basal uptake values see Table 1. ^*P<0.05 vs insulin effect in the absence of wortmannin.