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Uncoupling of eNOS in aortic rings from control rats and diabetic rats (STZ) treated either with placebo or sildenafil (5 mg kg−1 day−1 for 2 weeks) was assessed by eNOS dimer/monomer ratio following low temperature SDS-PAGE (a) *P<0.05 vs Control; #P<0.05 vs STZ-placebo. Macrophage infiltration into aortae was examined by immunohistochemistry for CD68+ cells (b). eNOS, endothelial NO synthase; SDS-PAGE, sodium dodecyl sulphate-polyacrylamide gel electrophoresis; STZ, streptozotocin.
