Skip to main content
. 2007 Dec 17;153(5):870–878. doi: 10.1038/sj.bjp.0707634

Figure 4.

Figure 4

Effect of MAPK inhibitors on ICAM-1 and VCAM-1 expressions. (a) HT29 cells were pretreated with SB202190 or SP600125 (10 μM) for 30 min and then incubated from time 0 to 8 h. At the time indicated, cells were processed for western blot analysis as described in the Methods section and analysed by densitometry. Data, normalised to β-actin, are expressed as percentage inhibition versus control, mean±s.e.m., n=3. Data were analysed by one-way ANOVA and the Dunnett's test (*P<0.05; **P<0.01 versus control). (b) The immunoblot shown is representative of three separate experiments. (c) HT29 cells were pretreated with SB202190 (0.001–10 μM) for 30 min and then plated for 4 h or directly incubated with SP600125 (0.001–10 μM) for 4 h and 30 min. Cells were processed for western blot analysis as described in the Methods section. The immunoblot shown is representative of four separate experiments.