Table 1.
Proteins of Escherichia coli TG1 host and engineered cells expressing GFPuv and chimeric VHbGFP identified by Mass spectrometry and peptide mass fingerprinting (PMF) analysis.
| Spot No. | Accession No. | Description | Calculated pI value | Nominal mass (Mr) | Protein score | Sequence Coverage (%) |
|---|---|---|---|---|---|---|
| 1, 37 | gi|75236921 | Aconitase B | 5.21 | 93996 | 170, 145 | 55, 64 |
| 2, 38 | gi|15830005 | 2-oxoglutarate dehydrogenase decarboxylase component | 6.04 | 105566 | 81, 92 | 43, 59 |
| 3 | gi|75197115 | Polyribonucleotide nucleotidyltransferase | 5.11 | 77110 | 90 | 62 |
| 4 | gi|15803853 | Elongation factor EF-2 | 5.24 | 77704 | 102 | 55 |
| 5 | gi|15799694 | Molecular chaperone DnaK | 4.83 | 69130 | 155 | 70 |
| 6 | gi|15830002 | Succinate dehydrogenase catalytic subunit | 5.85 | 65008 | 99 | 42 |
| 7 | gi|15834378 | Chaperonin GroEL | 4.85 | 57464 | 214 | 68 |
| 8 | gi|38704234 | Aspartate ammonia-lyase | 5.19 | 52950 | 82 | 44 |
| 9, 10 | gi|15801728 | Aldehyde dehydrogenase | 5.07 | 52377 | 147, 86 | 63, 53 |
| 11, 40, 65 | gi|442946 | Glycerol kinase | 5.36 | 56349 | Range 84-123 | 52 |
| 12, 26, 42, 43, 52, 53, 54 | gi|41936 | Tryptophanase | 5.88 | 53098 | Range 80-143 | Range 41-60 |
| 13, 27, 44, 66 | gi|33383669 | Isocitrate dehydrogenase | 5.33 | 43192 | Range 101-127 | Range 35-55 |
| 14, 28, 29, 45, 55, 56 | gi|16975437 | Enolase, chain A | 5.32 | 45552 | Range 80-127 | Range 54-64 |
| 15, 16, 46 | gi|124532037 | Translation elongation factor Tu | 5.01 | 40514 | Range 103-122 | 68 |
| 17 | gi|14277926 | Transaldolase B, chain A, | 5.18 | 35193 | 95 | 44 |
| 18, 75, 76 | gi|16130152 | Outer membrane porin protein C | 4.58 | 40343 | Range 141-176 | Range 69-77 |
| 19 | gi|15802193 | Glyceraldehyde-3-phosphate dehydrogenase | 6.61 | 35681 | 101 | 53 |
| 20, 47, 77 | gi|9507742 | Outer membrane protease precursor [Plasmid F] | 5.91 | 35477 | Range 98-129 | Range 61-72 |
| 21 | gi|15800433 | Succinyl-CoA synthetase alpha subunit | 6.32 | 30044 | 83 | 60 |
| 22, 34, 79, 92 | gi|75196144 | Fructose/tagatose bisphosphate aldolase | 5.87 | 31021 | Range 80-96 | Range 62-65 |
| 23, 35, 36, 51, 61, 80, 93 | gi|145306442 | Beta-lactamase | 5.35 | 31247 | Range 147-197 | Range 49-80 |
| 24a | gi|1000056 | Ribose-binding protein complexed with Beta-D-Ribose | 5.99 | 28457 | 101 | 70 |
| 24b | gi|26246721 | Phosphoglyceromutase | 6.27 | 29204 | 97 | 62 |
| 25 | gi|75209666 | Glutathione S-transferase | 5.22 | 24328 | 91 | 61 |
| 30 | gi|226907 | Malate dehydrogenase | 5.61 | 32417 | 93 | 55 |
| 31, 32, 89, 90 | gi|15800816 | Outer membrane protein 3a | 5.99 | 37292 | Range 80-88 | Range 42-45 |
| 33, 60 | gi|230520 | D-Galactose-D-Glucose Binding Protein (GGBP) | 5.25 | 33347 | 111, 189 | 62, 70 |
| 39, 64 | gi|15829780 | Heat shock protein 90 | 5.09 | 71378 | 220, 80 | 49, 42 |
| 41 | gi|15804332 | ATP synthase subunit B | 4.90 | 50351 | 86 | 44 |
| 48, 78, 91 | gi|2293126 | Beta-lactamase | 5.93 | 31730 | Range 138-180 | Range 68-75 |
| 49, 50, 62, 63, 67, 68, 69, 73, 81, 83, 84, 86 | gi|1490529 | GFPuv | 5.8 | 26893 | Range 99-121 | Range 53-60 |
| 57, 85 | gi|26249935 | Elongation factor Tu | 5.25 | 44993 | 112, 108 | 65, 68 |
| 58, 88 | gi|75237909 | Malate/lactate dehydrogenases | 5.61 | 32502 | 97, 150 | 68, 68 |
| 59 | gi|13399487 | L-Asparaginase | 5.66 | 34671 | 84 | 54 |
| 70a | gi|1490529 | GFPuv | 5.8 | 26893 | 85 | 65 |
| 70b | gi|114816 | Bacterial hemoglobin (Soluble cytochrome O) | 5.31 | 15821 | 81 | 50 |
| 71, 72 | gi|1942721 | Chain A, Elongation Factor Complex Ef-TuEF-Ts | 5.22 | 42321 | 106, 102 | 58, 65 |
| 74, 87 | gi|15833050 | Fructose-bisphosphate aldolase | 5.52 | 39351 | 107, 122 | 42, 48 |
| 82 | gi|26250698 | ATP-dependent protease ATP-binding subunit | 5.24 | 49664 | 92 | 41 |
Note: Protein scores greater than 80 are significant (p<0.05). Protein scores and sequences coverage of multiple protein spots were given as the range of values.