Table 1. kcat/Km values for cleavage of ACC-linked tetrapeptides, in the format P4-P3-P2-Arg-ACC, by rFVIIa in the presence of sTF.
Values are means±S.D. The assay was carried out in 50 mM Hepes (pH 7.4), 100 mM NaCl, 5 mM CaCl2 and 0.01% Tween 80. n.d., not determined.
| kcat/Km (mM−1·s−1) | |||||
|---|---|---|---|---|---|
| P2 residue | P3 residue… | Alanine | Leucine | Tyrosine | Tryptophan |
| Threonine | |||||
| P4 residue | |||||
| Asparagine | 2.6±0.04 | 13.8±0.1 | n.d. | 19.6±0.4 | |
| Phenylalanine | 19.4±0.5 | 12.2±0.5 | 24.5±0.2 | 24.6±0.3 | |
| Tryptophan | 49.8±0.6 | 25.8±0.3 | 44.0±0.4 | 23.7±0.2 | |
| Leucine | |||||
| P4 residue | |||||
| Asparagine | 1.1±0.005 | 5.7±0.07 | 9.8±0.04 | 11.2±0.1 | |
| Phenylalanine | 9.7±0.1 | 7.8±0.2 | 7.4±0.1 | 7.2±0.2 | |
| Tryptophan | 19.2±0.3 | 11.7±0.08 | 18.0±0.08 | 6.1±0.04 | |