Table 2. k_cat/K_m values for cleavage of S-2288 and Trp-Ala-Thr-Arg-ACC, as well as activation of FX, by rFVIIa and the FVIIa variants in the presence of sTF.

Values are means±S.D. The assay was carried out in 50 mM Hepes (pH 7.4), 100 mM NaCl, 5 mM CaCl₂ and 1 mg/ml BSA.

	kcat/Km (mM−1·s−1)
FVIIa variant	S-2288	Trp-Ala-Thr-Arg-ACC	FX
rFVIIa	26.12±2.77	44.66±0.11	550±30
T239A	5.45±0.15	28.59±0.08	69±5
T239G	2.40±0.036	17.59±0.10	23±0.6
T239I	19.71±2.26	29.01±0.07	461±12
T239Y	2.47±0.18	6.08±0.02	74±5