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. 2007 Jul 13;405(Pt 3):417–428. doi: 10.1042/BJ20070400

Figure 8. Effects of fibulin-5 on SMC phosphorylation of PDGFRs.

Figure 8

(A) A human phospho-RTK array was used to examine SMC receptor signalling when plated on 500 nM full-length fibulin-5 (F5) in the presence or absence of the β1 integrin-activating mAb TS2/16, or on fibronectin (FN), or on tissue culture plastic (TCP). Cells on fibulin-5 showed no detectable PDGFR signalling, and only traces of EGF receptor (EGF R) signalling. However, cells on fibulin-5 in the presence of TS2/16, or on fibronectin, showed increased signalling, especially by PDGFRα and EGF receptor. (PDGF-AA and PDGF-BB both signal through PDGFRα.) (B) Normalized relative quantification of signalling by PDGFRs and EGF receptor (EGF R) from RTK assays. The mean pixel densities of duplicate RTK spots were normalized against duplicate phosphotyrosine-positive control spots.