Figure 3. Inhibition of HNE by WT and pre-elafin mutants.

The residual activity (Fractional Activity) of HNE (10 nM) was assayed at 25 °C following a 30 min incubation in the presence of the indicated concentrations of inhibitor. The inhibitors used were: ●, pre-elafin; ■, pre-elafin^M25K; dashed line, pre-elafin^M25G. The lines are the best fit of eqn (1) to the experimental data. Note that no experimental points are shown for pre-elafin^M25G as the smallest concentration showing a significant inhibition was 350 nM.