Table 1. Plasmids used in the present study.
CMV, cytomegalovirus.
| Plasmid | Description | Source or reference |
|---|---|---|
| pCMV-Tag2b | CMV promoter, N-terminal FLAG tag | Stratagene |
| pCMV-hCdc34(Wt) | Human wild-type Cdc34 | The present study |
| pCMV-hCdc34(S231T) | (S231T) | The present study |
| pCMV-hCdc34[S(all4)A] | (S203A/S222A/S231A/S236A) | The present study |
| pCMV-hCdc34(S203) | (S222A/S231A/S236A) | The present study |
| pCMV-hCdc34(S222) | (S203A/S231A/S236A) | The present study |
| pCMV-hCdc34(S236) | (S203A/S222A/S231A) | The present study |
| pCMV-hCdc34(3Asp) | (S203D/S222D/S231D) | The present study |
| pET15b | N-terminal His6 tag, T7 promoter | Novagen |
| pET15b-hCdc34(Wt) | Human wild-type Cdc34 | The present study |
| pET15b-hCdc34(3Ala) | (S203A/S222A/S231A) | The present study |
| pET15b-hCdc34(3Asp) | (S203D/S222D/S231D) | The present study |
| pMS325 | ARS1/CEN4, LEU2, PNOP1, N-terminal FLAG tag, derivative of pNOPPATA1L | The present study |
| pMS325-hCdc34(Wt) | Human wild-type Cdc34 | The present study |
| pMS325-hCdc34(3Ala) | (S203A/S222A/S231A) | The present study |
| pMS325-hCdc34(3Asp) | (S203D/S222D/S231D) | The present study |
| pESC-TRP | 2μ, TRP1, PGAL1-10, N-terminal FLAG tag | Stratagene |
| pESC1 | Yeast wild-type Cdc34 in pESC-TRP | [23] |
| pESC1(1-252) | Truncation of C-terminal amino acids 253–295 | The present study |
| pESC1(1-200) | Truncation of C-terminal amino acids 201–295 | The present study |
| pESC1(S(all6)A) | (S207A/S216A/S263A/S268A/S282A/S292A) | The present study |
| pESC1(S207) | (S216A/S263A/S268A/S282A/S292A) | The present study |
| pESC1(S216) | (S207A/S263A/S268A/S282A/S292A) | The present study |
| pESC1(S263) | (S207A/S216A/S268A/S282A/S292A) | The present study |
| pESC1(S268) | (S207A/S216A/S263A/S282A/S292A) | The present study |
| pESC1(S282) | (S207A/S216A/S263A/S268A/S292A) | The present study |
| pESC1(S292) | (S207A/S216A/S263A/S268A/S282A) | The present study |
| pESC1(2Ala) | (S207A/S216A) | The present study |
| pESC1(2Asp) | (S207D/S216D) | The present study |
| pESC1(2Glu) | (S207E/S216E) | The present study |
| pET15b-yCdc34(Wt) | Yeast wild-type Cdc34 | The present study |
| pET15b-yCdc34(2Ala) | (S207A/S216A) | The present study |
| pET15b-yCdc34(2Asp) | (S207D/S216D) | The present study |
| pET15b-yCdc34(2Glu) | (S207E/S216E) | The present study |
| pET21(+)-yCdc34(F72V) | N-terminal His6 tag, T7 promoter, (F72V) | [24] |
| pRS415 | CEN6, LEU2 | Stratagene |
| pMS384 | Yeast CDC34 promoter in pRS415 | The present study |
| pMS384-yCdc34(Wt) | Yeast wild-type Cdc34 | The present study |
| pMS384-yCdc34(2Ala) | (S207A/S216A) | The present study |
| pMS384-yCdc34(2Asp) | (S207D/S216D) | The present study |
| pMS384-yCdc34(2Glu) | (S207E/S216E) | The present study |
| pMS384-yCdc34(F72V) | (F72V) | The present study |
| pRSETb | N-terminal His6 tag, T7 promoter | Invitrogen |
| pRSETb-Sic1 | Sic1 | The present study |