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. 2007 Jul 13;405(Pt 3):605–615. doi: 10.1042/BJ20061797

Figure 2. NF-κB-binding sites in the hBD-2 promoter are essential, but not sufficient, for the IκB-ζ-mediated transcriptional activation.

Figure 2

(A) A schematic illustration of the hBD-2 promoter. (B–D) HEK-293 cells were transfected with indicated reporter plasmids with a 5′-truncation or point mutations of the hBD-2 promoter (B and C), or pNF-κB-Luc harbouring four canonical NF-κB-binding sites or phBD-2-κB-Luc in which the NF-κB-binding sites of the hBD-2 promoter were substituted for those of pNF-κB-Luc (D), together with the expression plasmid for the NF-κB subunit p65 or p50 and the IκB-ζ expression plasmid. The total amount of DNA was kept constant with an empty vector. At 24 h after transfection, the cells were lysed and luciferase activities were measured. Results are means±S.E.M. for duplicate samples, representative of at least two independent experiments.