Figure 3. NF-κB- and C/EBP-binding sites in the hBD-2 promoter are core elements for the IκB-ζ-mediated transcriptional activation.

(A–C) HEK-293 cells were transfected with the hBD-2 promoter reporter plasmids with an internal deletion (A and C) or point mutations in the C/EBP-binding site (B), together with the expression plasmid for the NF-κB subunit p65 or p50 and the IκB-ζ expression plasmid. The total amount of DNA was kept constant with an empty vector. At 24 h after transfection, the cells were lysed and luciferase activities were measured. Results are means±S.E.M. for duplicate samples, representative of at least two independent experiments.