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. 2007 Jul 13;405(Pt 3):617–623. doi: 10.1042/BJ20061778

Figure 1. JNK is required for cisplatin-induced apoptosis via p73.

Figure 1

SP600125 (A) and JNK siRNA (C) block p73α-mediated apoptosis induced by cisplatin. Cells were stained with DAPI and apoptotic cells were counted on the basis of condensed nuclei; for the example depicted in (B) the white arrows point to apoptotic cells. The data represent the means for three independent experiments in which at least 200 cells were counted per condition in a blind manner. (A) H1299/p73α cells were treated overnight with or without ponasterone A (5 μM) to induce p73α expression (−p73 and +p73). Cells were pre-treated with SP600125 (10 μM) for 1 h prior to treatment with cisplatin (25 μM) for 24 h. (C) H1299/p73α cells were transfected with either non-specific control siRNA or JNK1/2 specific siRNA. At 24 h post-transfection ponasterone A was added overnight to induce p73α expression. Cells were then treated with cisplatin (25 μM) for 24 h and analysed as in (A).