Table 2. Oligonucleotides used in the back-mutations from PK1A2 endosialidase.
| Name | 5′–3′ Sequence | |
|---|---|---|
| Back-mutations | Y417H | CCACATAATGTGCATCATACTAC |
| Y417H | AAATCTTAGTGACTCCCAAGTC | |
| D489N | GACATTGTGAACTCTAGTGTAGG | |
| D489N | ACCCTGATAGATTTGGTCTGTG | |
| N-terminal deletions | PK1A-A | TCAGGTAAGAGAATTGACGG |
| PK1A-B | GCTTGGCCTCAAGACAAAGC | |
| PK1A-C | CTGCATCCAGATTACCCTAC | |
| PK1A-D | GGTGGTATCACTAAGGCTGC | |
| STALKEND | CGTGTACAGCTTATCTAACC | |
| C-terminal deletions | PK1A-1 | TTCGGATGCAAAAGCAGCTAC |
| PK1A-2 | CTTATGAGTGGCATCAGACG | |
| PK1A-3 | AGAAGTACTAGAACCTGCTCC | |
| PK1A-4 | TGAGCGAATATTAGCACTTGTAC | |
| STOP-pri | AGCTGAGCTTGGACTCCTG |