Table 2. Oligonucleotides used in the back-mutations from PK1A2 endosialidase.
Name | 5′–3′ Sequence | |
---|---|---|
Back-mutations | Y417H | CCACATAATGTGCATCATACTAC |
Y417H | AAATCTTAGTGACTCCCAAGTC | |
D489N | GACATTGTGAACTCTAGTGTAGG | |
D489N | ACCCTGATAGATTTGGTCTGTG | |
N-terminal deletions | PK1A-A | TCAGGTAAGAGAATTGACGG |
PK1A-B | GCTTGGCCTCAAGACAAAGC | |
PK1A-C | CTGCATCCAGATTACCCTAC | |
PK1A-D | GGTGGTATCACTAAGGCTGC | |
STALKEND | CGTGTACAGCTTATCTAACC | |
C-terminal deletions | PK1A-1 | TTCGGATGCAAAAGCAGCTAC |
PK1A-2 | CTTATGAGTGGCATCAGACG | |
PK1A-3 | AGAAGTACTAGAACCTGCTCC | |
PK1A-4 | TGAGCGAATATTAGCACTTGTAC | |
STOP-pri | AGCTGAGCTTGGACTCCTG |