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. 2007 Jul 13;405(Pt 3):489–494. doi: 10.1042/BJ20070174

Figure 3. DNA binding to His–centerin.

Figure 3

(A) The Figure shows a gel retardation assay in which 1.5 μg of linearized plasmid DNA was incubated with 0 μg (lane 1), 2 μg (lane 2), 10 μg (lane 3) and 20 μg (lane 4) of His–centerin for 20 min prior to 1% agarose gel electrophoresis and visualization using ethidium bromide. (B) The Figure shows the effects of salt concentration on binding of His–centerin to DNA. Lane 1 shows 1.5 μg of DNA alone. Lanes 2–5 contain 1.5 μg of DNA incubated with increasing concentrations of NaCl (lane 2, 160 mM; lane 3, 200 mM; lane 4, 250 mM; and lane 5, 300 mM). Lanes 6–9 show 1.5 μg of DNA incubated with 5 μg of His–centerin in the presence of increasing concentrations of NaCl (lane 6, 160 mM; lane 7, 200 mM; lane 8, 250 mM; and lane 9, 300 mM).