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Gel retardation assays using acid-native PAGE are shown. (A) His–centerin (10 μg) was incubated with 0, 2.5, 5, 10, 20 and 50 units/ml (lanes 1–6 respectively) unfractionated heparin. (B) His–centerin (10 μg) was incubated with 0, 2, 4, 6, 8, 10, 12 and 14 μg/ml (lanes 1–8 respectively) chondroitin sulfate.
