Figure 2. Cells lacking Bax and Bak are resistant to Bnip3-mediated mitochondrial dysfunction and cell death.

WT and Bax/Bak DKO MEFs were transfected with vector or Bnip3 for 24 h and (A) the ΔΨ_m was measured by TMRM fluorescence and (B) cell death was assessed by measuring plasma membrane permeability to YoPro-1. Results are means±S.E.M., n=3. (C) Expression of Bnip3 in WT and Bax/Bak DKO MEFs transfected with vector or Bnip3 for 24 h was determined by Western blot analysis.