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. 2007 Jul 13;405(Pt 3):591–596. doi: 10.1042/BJ20070231

Figure 1. PP2Cϵ associates with ASK1.

Figure 1

(A) Schematic diagram showing the SAPK signalling pathways. ATF2, activating transcription factor 2; MLK, mixed lineage kinase. (B) HEK-293 cells were transiently transfected with an expression plasmid encoding Myc–TAK1 or ASK1–Myc in the presence or absence of an expression plasmid encoding HA–PP2Cϵ. Proteins were immunoprecipitated (IP) from cell lysates with an anti-HA antibody (αHA); precipitates were immunoblotted (WB) with an anti-Myc antibody (αMyc). The cell lysates were also immunoblotted with anti-Myc or anti-HA antibodies. The results are from one of two reproducible experiments. (C) Proteins immunoprecipitated (IP) from mouse brain cell extracts with normal rabbit IgG (Cont) or an anti-PP2Cϵ antibody (αPP2Cϵ) were immunoblotted (WB) with an anti-ASK1 antibody (αASK1). The results are from one of two reproducible experiments.