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. 2007 Nov 14;408(Pt 2):149–159. doi: 10.1042/BJ20070677

Figure 1. SDS/PAGE patterns from a representative purification of maize THI3 protein overproduced in E. coli.

Figure 1

The electrophoresis was carried out in the Laemmli system [21] using a 10% resolution gel. Protein bands were visualized by Coomassie Brilliant Blue R-250 staining. Left lane: molecular mass standards; lane 1: transformed cell lysate before IPTG induction; lane 2: transformed cell lysate after IPTG induction for 16 h at 16 °C; lane 3: sample after chitin-affinity chromatography; lane 4: sample after Superdex-200 gel filtration.