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. 2007 Nov 14;408(Pt 2):149–159. doi: 10.1042/BJ20070677

Figure 3. Kinetic characteristics of HMP kinase activity of THI3.

Figure 3

THI3 has a dependence of HMP-P formation rate on concentrations of HMP and ATP substrates. The production of HMP-P was directly quantified by RP-HPLC with spectrophotometric detection. Ordinary hyperbolic fits are shown with Km and Vmax values of 7.46 μM and 20.5 nmol HMP-P per min per mg of protein respectively, for HMP concentration dependence, and of 6.27 μM and 17.4 nmol of HMP-P/min per mg of protein respectively, for the dependence on ATP concentration.