Table 1. Primers used in the present study.

Name of primer	Sequence	Description
MO1-F	TCGTGAAGACTGGGATGCT(TC)CC	Degenerate, forward primer
MO2-R	GTAGTC(CT)GCACCATCTTTCCA	Degenerate, reverse primer
ZTH51	GCTTCTTTCACATTTGGAGTGAC	5′-RACE RT primer
ZTH52	GTAGCGAGAGTAGATGGTCC	First 5′-RACE primer
ZTH53	GCTGGTGGACACCATGACC	‘Nested’ 5′-RACE primer
ZTH31	GCGTCCATGTTGGCCAATC	First 3′-RACE primer
ZTH32	CATACCAGCATGGGAAGTAC	‘Nested’ 3′-RACE primer
Adaptor	GACTCGAGTCGACATCG	Adaptor 3′- and 5′-RACE primer
ZTH1-F	GGTGGTCATATGACGTCCGTACCGCTAC	Forward primer for PCR of thi3 cloned into pTYB1 vector
ZTH1-R	GGTGGTTGCTCTTCCGCAAGACCTTGAGTGAGCATTCTTC	Reverse primer for PCR of thi3 cloned into pTYB1 vector
ZTH11-F	GGTGGTTGCTCTTCCAACGCCACCCTTGGCTTCCGCA	Forward primer for PCR of thi3 cloned into pTYB11 vector
ZTH11-R	GGTGGTCTCGAGCTAAGACCTTGAGTGAGCATTC	Reverse primer for PCR of thi3 cloned into pTYB11 vector
MutQ98L	GCTGTCACCGCGCTGAACACCGTCGG	Mutagenetic primer, replaces Gln98 with leucine
MutQ373L	GGCGCTACCATTGTCCTACTGAGAGAAAAAGACGCC	Mutagenetic primer, replaces Gln373 with leucine
MutM134K	GGTGAAAACAGGGAAGCTCCCTTCAGCTGG	Mutagenetic primer, replaces Met134 with lysine
MutT472D	GTGTCTTCCCGACCACGGACAAGGCAAACAATCCTACC	Mutagenetic primer, replaces Thr472 with aspartic acid
MutS444A	GGAAAAATCATCGGCGTCGCATGTAAGACCCTGG	Mutagenetic primer, replaces Ser444 with alanine