Figure 6. Extracellular localization of the GH5BG protein.

The epidermal layers of onion bulbs were transformed with (A) pMDC139-GH5BG and (B) control pMDC139 plasmid without insert by particle bombardment. After 48 h incubation at 25 °C in complete darkness, the transformed onion cells were stained overnight with X-Glu at 37 °C, and the location of blue-coloured product was observed by optical microscopy.