Table 1. The kinetics parameters of PAI-1, PAI-1R76E, PAI-2, uPA and uPA–serpin complexes binding to VLDLr, measured by SPR.
Analyte | Binding model | ka (M−1·s−1) | kd (S−1) | KDa (nM) | ϕ2 |
---|---|---|---|---|---|
PAI-1 | 1:1 | 9.90×104 (±6.83×104) | 4.17×10−3 (±1.19×10−3) | 51.8 (±21.9) | 8.12 |
PAI-1R76E | No binding | − | − | − | − |
PAI-2 | No binding | − | − | − | − |
uPA | Heterologous analyte | 8.00×104 (±1.08×104) | 1.32×10−2 (±0.01×10−2) | 209 (±25.1) | 13.9 |
5.48×104 (±0.74×105) | 1.50×10−3 (±0.13× 0−3) | 31.2 (±5.69) | |||
uPA–PAI-1 | Heterologous analyte | 1.50×105 (±0.20×105) | 1.30×10−2 (±0.10×10−2) | 84.8 (±1.69) | 19.6 |
1.00×105 (±0.40×104) | 1.30×10−4 (±0.18×10−3) | 1.51 (±0.27) | |||
uPA–PAI-1R76E | 1:1 | 7.79×104 (±0.84×104) | 7.08×10−4 (±2.01×10−4) | 9.95 (±3.53) | 8.2 |
uPA–PAI-2 | 1:1 | 1.73×105 (±0.62×105) | 5.61×10−4 (±0.98×10−4) | 4.68 (±0.90) | 1.9 |