Table 1. Repartition of sialidase activity.
Total NEU3 enzymatic activity in particulate (P) and soluble (S) fractions obtained from total cell membranes (T) of transfected COS-7 cells (MmNEU3–HA) was assayed under control and alkaline (carbonate extraction) conditions. Values, representative of three independent experiments, are expressed as total activity recovered in each fraction. Mock, COS-7 cells transfected with vector alone.
| Mock | MmNEU3–HA | Control | Carbonate extraction | |||
|---|---|---|---|---|---|---|
| T | T | P | S | P | S | |
| 4MU-NeuAc (nmol/h) | 15.7±1.8 | 125.7±20.8 | 110.7±14.1 | 8.1±2.8 | 0.4±0.2 | 0.3±0.2 |
| [3H]GD1a (μmol/h) | 73.5±7.8 | 1090.0±185.3 | 900.0±117.0 | 160.0±56.0 | 30.0±14.4 | 10.0±6.7 |