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. 2007 Nov 14;408(Pt 2):161–172. doi: 10.1042/BJ20070761

Figure 7. The NHB1 of Nrf1 redirects Nrf2 fusion proteins to the ER.

Figure 7

(A) COS-1 cells were transfected with expression constructs for chimaeric proteins containing the NTD and its mutants from Nrf1 fused to the N-terminus of the full-length Nrf2, as described in the Experimental section. Subcellular localization of these fusion proteins was determined by immuocytochemistry and confocal imaging as described in the legends for Figures 2 and 3. Scale bar=20 μm. (B) The transactivation activity of these fusion proteins was examined as described in the text. (C and D) The V5-tagged fusion proteins were separated by NuPAGE in a 7% polyacrylamide Tris/acetate buffer system (C) or 4–12% polyacrylamide gradient Bis/Tris buffer system (D).