Table 1. Primers used in the Y2H assays.
Protein regions encoded by cDNA PCR fragments were generated with the corresponding primers. The restriction enzyme sites are underlined.
| Primer name | Protein region | Direction | Oligonucleotide sequence (5′→3′) |
|---|---|---|---|
| PA24253 | Ameloblastin-(1–396) | Forward | CGCCATATGGTGCCGGCATTTCCTCAACAACCTGGG |
| PA24254 | Ameloblastin-(1–396) | Reverse | CGCGTCGACTCAGGGCTCTTGGAAACGCCATGC |
| PA27536 | Enamelin-(1–1236) | Forward | TTCTCGAGCCCGGGGCCATTCCAGATGCCAATGCCCCGA |
| PA27537 | Enamelin-(1–1236) | Reverse | ACGACCCCCAAAGCCGTGATATCC |
| PA24255 | Amelogenin-(1–180) | Forward | CCGGAATTCATGCCCCTACCACCTCATCCTGGA |
| PA24256 | Amelogenin-(1–180) | Reverse | CGCGTCGACTTAATCCACTTCTTCCCGCTTGGTCTTG |
| PA39335A | Amelogenin-(3–33) | Forward | GGAATTCCTACCACCTCATCCTGGAAGCCC |
| PA39335B | Amelogenin-(3–33) | Reverse | GGTCGACTCACGGCTGCCTTATCATGCTCTGG |
| PA39335C | Amelogenin-(155–179) | Forward | GGAATTCCCCCTGTCCCCCATTCTTCCTG |
| PA39335D | Amelogenin-(155–179) | Reverse | GGTCGACTCACACTTCTTCCCGCTTGGTC |
| INV48566044 | Amelogenin-(169–179) | Forward | GGAATTCCCAGCGACAGACAAGACCAAGC |
| INV48566043 | Amelogenin-(169–179) | Reverse | CCTGAGAAAGCAACCTGACCTACAGGA |
| INV47327732 | Amelogenin-(155–168) | Reverse | GGTCGACTCACCAAGCTTCCAGAGGCAGCTCAG |
| PA29499 | CD63-(1–238) | Forward | AGTGAATTCATGGCGGTGGAAGGAGGAATGAAATGTGTG |
| PA29500 | CD63-(1–238) | Reverse | CCTCGAGCTACATCACCTCGTAGCCACTTCTGATACTCTTCA |
| hCD63_103 | CD63-(103–205) | Forward | TGAATTCGCTGGCTATGTGTTTAGAGATAAGGTG |
| INV47327730 | CD63-(165–205) | Forward | TGAATTCGTCCCCGACTCCTGCTGC |
| INV47327734 | CD63-(165–205) | Reverse | GCTCGAGTCACACCAGCACATTTTTCCTCAGCC |
| PA39458A | LAMP1-(1–121) or -(1–386) | Forward | GCCCGGGTATGGCGCCCCGCTCTCCGC |
| PA39335F | LAMP1-(1–386) or -(342–386) | Reverse | GGGATCCTCAGATGGGGATCAGCGTGCTG |
| PA39458B | LAMP1-(1–121) | Reverse | GGGATCCTCAGTTATAAACAAAACTCATGAGCTGGACGC |
| PA39335H | LAMP1-(342–386) | Forward | GCCCGGGTGAGCACGTCCGTGTCACGAAG |
| PA39335I | LAMP1-(95–251) | Forward | GCCCGGGTAGAGGACATACACTCACTCTCAATTTC |
| PA39335J | LAMP1-(95–251) | Reverse | GGGATCCTCACGTCGTGTTGTCCTTCCTCTCATAG |
| PA39336A | LAMP1-(226–361) | Forward | GCCCGGGTGGCACCAACGGGACCTGC |
| PA39336B | LAMP1-(226–361) | Reverse | GGGATCCTCAAGCCTGGACCCACACTTTGAA |
| INV47327729 | LAMP1-(226–251) | Forward | TGAATTCGGCACCAACGGGACCTGC |
| INV47327731 | LAMP1-(226–251) | Reverse | GCTCGAGTCACGTCGTGTTGTCCTTCCTC |
| INV47327733 | LAMP2-(227–259) | Forward | TGAATTCAATGGCAATGATACTTGTCTGCTGGC |
| INV47327735 | LAMP2-(227–259) | Reverse | GCTCGAGTCAAGTTGTATTGGGGTTGATGTTAATAACTGAAGC |