Figure 6. Association of Imp4p with telomeres in vivo.

(A) Schematic presentation of URA3, ADH4 and ARO1 chromosomal loci monitored by ChIP [34]. The positions of the PCR-amplified DNA fragments are presented by solid bars. (B) Imp4p interacts with telomeres in vivo. Total yeast extract was immunoprecipitated with antibodies against Cdc13p or Myc. The immunoprecipitates were then subjected to multiplex PCR analysis. The PCR products were separated on a 2% agarose gel and stained with ethidium bromide. Sizes are indicated in bp. (C) Quantification of the experiments was conducted using densitometry-scanning of the signals. In each set of experiments, the TEL or ADH signal is normalized with ARO signals (TEL/ARO or ADH/ARO). Results are means±S.D. for four independent experiments. *P<0.05 compared with input control.