. 2007 Nov 28;408(Pt 3):327–333. doi: 10.1042/BJ20071017

Table 2. Parameters describing the effects of nucleotides on Co²⁺- and Mn²⁺-bound mtCBS-PPase.

		Co²⁺/Mg²⁺-activated enzyme		Mn²⁺/Mg²⁺-activated enzyme
Enzyme	Nucleotide	A_+L/A_−L (%)	K_d (μM)	A_+L/A_−L (%)	K_d (μM)	Source
Wild-type	AMP	3.7±0.4	0.22±0.02	20±4	1.4±0.2	Inhibition of activity (Figure 1)
	AMP	0.17±0.05				Effect on ATP activation
	ADP	0.8±0.3	0.012±0.001	21±2	0.033±0.004	Inhibition of activity (Figure 1)
	ADP	0.017±0.005				Effect on ATP activation (Figure 3)
	ATP	180±10	0.20±0.05	400±40	7.2±0.8	Inhibition of activity (Figure 1)
	ATP	0.16±0.01				Effect on AMP inhibition
	ATP	0.6±0.2				Effect on ADP inhibition (Figure 3)
	CDP	<10	150±20			Inhibition of activity
	UDP	<10	340±90			Inhibition of activity
Y169A	AMP	52±2	5.3±0.9			Inhibition of activity
	ADP	6.7±0.4	0.21±0.02			Inhibition of activity
	ATP	143±4	2.4±0.9			Inhibition of activity

Table 2. Parameters describing the effects of nucleotides on Co2+- and Mn2+-bound mtCBS-PPase.