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. 2008 Feb 8;9(3):273–279. doi: 10.1038/embor.2008.3

Figure 4.

Figure 4

Constitutive SUMO2 conjugation of NPM1 interferes with pre-ribosomal RNA processing. (A) HeLa cells expressing Flag-NPM1 or Flag-NPM1–SUMO2 from a tetracycline-inducible promoter were transfected with an siRNA duplex targeting NPM1 or a control siRNA. At 72 h after transfection, in vivo labelling and processing of the samples was carried out as described in Fig 3. (B) The signal intensities were quantified as described in Fig 3D. (C) Homogenous expression and localization of Flag-NPM1 or Flag-NPM1–SUMO2 was monitored by indirect immunofluorescence. EtBr, ethidium bromide; NPM1, nucleophosmin; siRNA, short interfering RNA.