Skip to main content
. 2008 Feb 8;9(3):260–266. doi: 10.1038/embor.2008.8

Figure 3.

Figure 3

Rept is an integral component of PRC1. (A) SDS–PAGE (silver stain) of PRC1. Protein bands are labelled according to Saurin et al (2001). (B) Western analyses of PRC1. NE: 20 μg nuclear extract; PRC1: 15 μl PRC1. (C) SDS–PAGE (Coomassie blue) of reconstituted PCCs from Sf9 cells expressing Flag-Polyhomeotic (F-Ph), Psc, Pc, dRing (PCC), and Pont (PCC-Pont) or Rept (PCC-Rept). *Nonspecific Sf9 protein. (D) Western analyses of Rept (left) and Pont (right) profiles during purification of PCCs shown in (C). In: 1.5 μl (15 μg) Sf9 nuclear extract; FT: 1.5 μl anti-Flag affinity column flow through; El: 3 μl purified complex. PCC, PRC1 core complex; Pont, Pontin; Psc, Posterior sex combs; Rept, Reptin; SDS–PAGE, SDS–polyacrylamide gel electrophoresis.